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«ABSTRACT BOOK XXVIIth EUCARPIA SYMPOSIUM ON IMPROVEMENT OF FODDER CROPS AND AMENITY GRASSES Copenhagen (Denmark), 19th to 23rd August 2007 th XVII ...»

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European Association for Research on Plant Breeding

Europäische Gesellschaft für Züchtungsforschung

Association Européenne pour l'Amélioration des Plantes

Abstract

BOOK

XXVIIth EUCARPIA SYMPOSIUM ON IMPROVEMENT OF FODDER

CROPS AND AMENITY GRASSES

Copenhagen (Denmark), 19th to 23rd August 2007

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XVII EUCARPIA SYMPOSIUM ON IMPROVEMENT OF FODDER

CROPS AND AMENITY GRASSES, Copenhagen (Denmark), 2007 th

XVII EUCARPIA SYMPOSIUM ON IMPROVEMENT OF FODDER

CROPS AND AMENITY GRASSES, Copenhagen (Denmark), 2007 Programme

Description of exhibitors

Abstracts for oral presentations

Poster Abstracts

List of participants

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XXVII EUCARPIA SYMPOSIUM ON IMPROVEMENT OF FODDER

CROPS AND AMENITY GRASSES, Copenhagen (Denmark), 2007 Programme Sunday, August 19 18:00 - 20:00 Registration, poster mounting, welcome reception Monday, August 20 7:30 Registration, poster mounting

Opening session:

8:30 Ian Max Møller, DJF, Research director Genetics and Biotechnology 8:45 Beat Boller, ART, Chairman EUCARPIA Fodder Crop and Amenity Grass section

Session 1:

Seed production Sponsored by

Chairpersons:

Birte Boelt, Ulrich Posselt 9:00 Anders Mondrup, DLF Limitations in seed production 9:30 Birte Boelt, DJF Seed yield components and their contribution to yield 10:00 Coffee break and poster viewing 10:30 Danny Thorogood, IGER Towards resolving vegetative and reproductive growth conflicts in turfgrasses 11:00 Grzegorz Zurek, IHAR Relation between seed yield potential and turf quality in Poa pratensis L.

11:20 Susanne Barth, TEAGASC Variation in seed yield and other morphological traits in a collection of Irish Lolium perenne ecotypes and bred varieties 11:40 Bruno Studer, DJF Genetic characterisation of seed yield and its components in perennial ryegrass Lolium perenne L.) 12:00 Peter Button, UPOV New developments in the international unio

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14:00 Thomas Lübberstedt, DJF EU-project “Development of allele-specific markers for sustainable grassland improvement (GRASP)”: overview

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15:30 Coffee break and poster viewing 16:00 Philippe Barre, INRA Association studies using synthetic varieties: case study of GAI gene and leaf length in perennial ryegrass 16:20 Lesley Turner, IGER Changes in allele frequency during phenotypic selection for leaf water-soluble carbohydrate (WSC) in an experimental population of perennial ryegrass 16:40 Roland Kölliker, ART Genetic characterisation of persistence in red clover (Trifolium pratense L.)

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17:00 - 18:30 Plenary: conclusions from the three workshops 18:30 - 19:00 Business meeting for EUCARPIA members / Poster removal 19:00 Free evening / Copenhagen tours Wednesday, August 22 8:00 From LIFE: Excursion to Tystofte (variety testing) and Research Centre Flakkebjerg

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9:00 René Gislum, DJF NIRS and chemometrics – exploration of grass forage quality 9:20 Kevin Jensen, USDA Forage quality of irrigated pasture species as affected by irrigation rate

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Roskilde, Denmark http://www.dlf.dk With an estimated market share of 20% worldwide, DLFTRIFOLIUM is the world’s biggest seed company within the breeding, production, sale and marketing of cool season clover and grass seed species.

The production of clover and grass seeds is mainly situated in Denmark, as the climate is favourable here and the growers are highly experienced in seed production. The company’s strong position on the world market for clover and grass seeds can be attributed to an efficient production and logistics function, where the seeds are cleaned on modern machinery and the quality of the product is documented via analyses in our own ISTA-accredited and authorised laboratories.

Barenbrug Royal Barenbrug Group, Oosterhout, The Netherlands www.barenbrug.com The Royal Barenbrug Group is a globally operating grass seed company. We specialise in plant breeding, seed production and international marketing of forage and turf grasses.

Research and Development in all major climatic zones around the world has resulted in a unique product line of high-quality speciality varieties. The company participates in research alliances with major universities and institutes. New innovations include the breakthrough of unique food-quality improving characteristics of grass in the production of dairy products.

CLF PlantClimatics GmbH Emersacker, Germany www.plantclimatics.de CLF Plant Climatics is the European market leader in the supply of plant growth and entomology chambers. As the exclusive partner of Percival Inc. in Europe we offer a broad spectrum of chambers from a compact LED chamber to a large area multipurpose Plant Growth Chamber.

We take care to listen and understand what you need before offering advice on which chamber could be most suitable for your research work. All the chambers we supply are installed and tested on site by our own fully trained technicians - all you need to do is open the door of the chamber and put your plants in.





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Doriane Scientific Software Solutions www.doriane.com DORIANE publishes LABKEY™, the core concept of this software package is the management of experiment cycle on live organism for research teams who requires sharing, security and tracking at the level of the Research Department.

LABKEY™ is made of two product lines based on one integrated data architecture, one for the management of organisms, their genetic properties and market qualities, the other for the management of high throughput analysis and molecular markers production.

Breeding companies and vegetal production agronomic department working on farm, vegetable and flower crops use LABKEY™.

Doriane has the recognized know-how to drive, with the IT customer team, the whole installation process and has many testimonies of participation to increase the efficiency of industrial Research Department having stations and laboratories in several countries.

LI-COR Biosciences www.licor.com The LI-COR 4300 DNA Analysis System, is a third generation infrared fluorescence detection system ideally suited to the exacting demands of functional genomics especially TILLING® (reverse genetics), Ecotilling (SNP research), and AFLP®. LICOR photosynthesis, carbon dioxide analysis and light measurement instruments are recognized worldwide for standard-setting innovation.

WINTERSTEIGER AG

Ried, Austria www.wintersteiger.com WINTERSTEIGER provides solutions for the mechanisation of agricultural research. That means complete product lines of machinery in a stable modular system with the option of transmitting the data for the purpose of research, breeding, yield trials and multiplications of all crops i.g. cereals, rice, maize, sunflowers, oil seed, legumes, fodder plants, vegetable seeds, clover and various grasses or special crops.

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EURO GRASS Breeding GmbH & Co. KG Lippstadt, Germany www.eurograss.com EURO GRASS Breeding is one of the leading companies in forage and turf grass breeding as well as in small seeded forage legumes. The main species are perennial ryegrass, Italian and annual ryegrass, timothy, meadow fescue, cocksfoot, kentucky bluegrass, red fescue and different clover species. International marketing and sales are carried out by Euro Grass, a joint venture of Deutsche Saatveredelung AG (Germany) and Hunsballe Frø (Denmark). Combining the strengths of these partners a powerful breeding network, large seed multiplication and efficient structures in sales, logistics and other services are built up to meet the customers’ needs.

Invitrogen www.invitrogen.com Invitrogen provides products and services that support academic and government research institutions and pharmaceutical and biotech companies worldwide in their efforts to improve the human condition. Invitrogen provides essential life science technologies for disease research, drug discovery, and commercial bio-production. Known for quality and consistency, Invitrogen’s products and services accelerate discovery by providing optimized, ready-to-use tools and processes. That means faster experimental results, as well as a better understanding of the molecule, system, or organism you’re studying. For more information about Invitrogen, visit www.invitrogen.com Medinova Scientific A/S www.medinova.dk Medinova Scientific A/S mainly operates within four market segments: Cell Biology, Molecular Biology, Haematology and Microplate Technology.

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Partec GmbH Münster, Germany www.partec.com Since more than 20 years Flow Cytometry plays an important role in Agroscience and Plant Breeding worldwide. The uniqueness of flow cytometric ploidy and gemome size analysis made FC an indispensable tool for research driven as well as commercial plant breeders. Flow cytometric DNA analysis allows monitoring DNA content and Ploidy level of high numbers of individuals at an early stage of plant development.

Partec GmbH contributes to this field of application since its early beginning through the development of dedicated, robust and reliable instrumentation and reagent protocols and can claim today to be market leader in the use of flow cytometry in these disciplines.

The Danish Agricultural Council Copenhagen, Denmark www.landbrugsraadet.dk The Danish Agricultural Council (the DAC) is the leading special-interest organisation for farmers and the food industry in Denmark. In 2006 our members exported agricultural products and equipment in the amount of EUR 14 billion and employed a total of 180,000 people.

SIGMA-ALDRICH

www.sigmaaldrich.com Produces and sells a broad range of biochemicals, organic and inorganic chemicals and related products.

Abstracts for oral presentations Limitations in seed production Mondrup, A.

DLF Trifolium A/S Seed yield components and their contribution to yield Boelt, B.

University of Aarhus, Faculty of Agricultural Sciences, Department of Genetics and Biotechnology, Research Centre Flakkebjerg, DK-4200 Slagelse, Denmark.

Tel: +45 89993500; fax: +45 89993501; E-mail: Birte.Boelt@agrsci.dk The yield components in grass seed crops are identified as: The number of reproductive tillers, the number of florets per tiller and their success in producing a seed (floret site utilisation, FSU) and seed weight.

The majority of species in grass seed production in Denmark require a dual flower induction to initiate reproductive growth, and the traditional Danish production system is to establish grass seed crops undersown in at cover crop (companion crop). The management of these double crops production systems has attained much focus in research i.e. determination of optimal cover crop species, seed rate, row distance, nitrogen fertilisation rates and distribution between autumn and spring etc. Data from these experiments have shown that in slow establishing species as smooth stalked meadow grass (Poa pratensis, L.) and red fescue (Festuca rubra, L.) a low number of reproductive tillers may restrict the seed grower in obtaining the optimum seed yield. However, due to the large number of experiments, knowledge is available to avoid a low number of reproductive tillers to restrict seed yield.

In a number of grass seed crops cultivation practice includes the application of a growth regulator to avoid lodging before flowering. It is assumed that grass seed crops without any lodging at flowering have a higher FSU and hence a higher seed yield potential. Thousand seed weight (TSW) is normally determined in the pure seed lot, and often only very small variation is found within the same variety. This may be due to the fact that some seeds are lost due to shedding before harvest, some are lost in the harvest operation and some are lost during cleaning. In general it is assumed that the seeds lost during harvest and cleaning operations are small ‘empty’ seeds, however, experiments are initiated to examine to what extent seed weight may be increased by cultivation techniques.

Towards Resolving Vegetative and Reproductive Growth Conflicts in Turfgrasses Thorogood, D. and Armstead, I.P.

Department of Plant Genetics and Breeding, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion, SY23 3EB, United Kingdom.

Turf quality is entirely dependent on vegetative characteristics, yet to produce a commercially viable turfgrass variety, its seed production potential must be optimised. However selection for seed production traits that are dependent on preferable allocation of resources from vegetative to reproductive organs will inevitably be negatively correlated with turf performance. The proportion of florets that produce a seed, and seed retention are two reproductive traits that are independent of vegetative growth performance traits and are therefore worth targeting. This paper focuses on seed set in perennial ryegrass for which there is considerable variation. Our approach has been to simply identify major QTL in mapping families that influence seed setting ability. In particular, we wanted to know whether the QTL identified were determined by resource allocation factors, that might suggest some compensatory mechanisms which would lead to no increase in overall seed yield potential, or, more significantly, genetic breakdown of gamete/embryo development processes. By comparing orthologous regions in rice, we also wished to identify putative orthologues of genes underlying these QTL, the physiological basis of which may already be known.

Relation Between Seed Yield Potential and Turf Quality in Poa pratensis L.

Prończuk S., urek G.

Plant Breeding and Acclimatization Institute, Radzików, 05-870 Błonie, POLAND The relation between seed yield and turf quality was disputable from many years. Also search for good ‘marker’ traits for seed yield and turf quality predicting is still in progress.



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